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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become observed in several tumors, which results in chromosome instability, and therefore could contribute to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential function in promoting mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually explained. Two extra splice variants were being documented but comprehensive nucleotide sequence information and facts is just not however readily available.
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Blocking peptides are peptides that bind exclusively on the target antibody and block antibody binding. These peptide normally incorporates the epitope acknowledged because of the antibody.
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This information outlines more feasible optimization of mobile and immunoassay parameters to guarantee the absolute best outcomes are...
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This guidebook outlines even more doable optimization of mobile and immunoassay parameters to guarantee the very best success are...
Antibodies sure to the blocking peptide now not bind into the epitope over the concentrate on protein. By evaluating the staining with the blocked antibody compared to the antibody by itself, one can see which staining is unique.
Focus on Information and facts Cyclin E belongs to the really conserved cyclin loved ones, whose members are characterised by a extraordinary periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Distinctive cyclins show distinctive expression and degradation designs which contribute into the temporal coordination of each and every mitotic function. Cyclin E varieties a fancy with and functions for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and is also degraded as cells development by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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